Key features and details
- Rabbit polyclonal to ZNF169
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZNF169 antibody
DescriptionRabbit polyclonal to ZNF169
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: HepG2 and HeLa whole cell lysate. IHC-P: Human colon cancer tissue and small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 68, 45 kDa (predicted molecular weight: 68 kDa).|
RelevanceZNF169 belongs to the krueppel C2H2 type zinc finger protein family. It may be involved in transcriptional regulation. It is highly expressed in kidney, weakly expressed in heart, liver, spleen, and small intestine. Not expressed in adult brain or spinal cord. ZNF169 contains thirteen C2H2 type zinc fingers and one KRAB domain.
- MGC51961 antibody
- Zfp169 antibody
- Zinc finger protein 169 antibody
- Znf169 antibody
All lanes : Anti-ZNF169 antibody (ab225924) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 45,68 kDa why is the actual band size different from the predicted?
Paraffin embedded human colon cancer tissue stained for ZNF169 with ab225924 (1/100 dilution) in immunohistochemical analysis.
Paraffin embedded human small intestine tissue stained for ZNF169 with ab225924 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225924 has not yet been referenced specifically in any publications.