Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.0625 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Information by UniProt
- PIGX antibody
- Zinc finger protein 177 antibody
- ZN177_HUMAN antibody
- ZNF177 antibody
Lane 1 : marker
Lane 2 : Anti-ZNF177 antibody (ab50718) at 0.0625 µg/ml
Lane 2 : Transfected 293T cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab50718 has not yet been referenced specifically in any publications.