Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    Tested in peptide ELISA. Antibody detection limit dilution 1/2000.
    Western Blot: Approx. 55-60kDa and 80-85kDa bands observed in A431 lysate at 1µg of primary. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of ~73KDa according to NP_009079.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    ab4838 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - citrate buffer
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Shao-ming Wang

    Verified customer

    Submitted Jul 12 2012

    Answer

    DISCOUNT CODE: XXXX

    Expiration date: 05-Sep-12

    I am very pleased to hear you would like to accept our offer and test ab4838 in mouse species with IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with IHC-Fr in Ms species and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    We have a special feedback reward system for Fast-track products: we will award 950 Abpoints to the first researcher who sends us positive feedback (including an image and details of your protocol). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 Abpoints.
    Other useful positive and negative feedback will be rewarded with the standard amount of Abpoints for submitting an Abreview (please refer to Abreviews terms and conditions for more detailed information, see https://www.abcam.com/abreviews).

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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