Overview

  • Product name
    Anti-ZNF185 antibody
  • Description
    Rabbit polyclonal to ZNF185
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ZNF185 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O15231

  • Positive control
    • Human lung carcinoma tissue and MDA-MB231 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab175603 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 74 kDa.

Target

  • Function
    May be involved in the regulation of cellular proliferation and/or differentiation.
  • Tissue specificity
    Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain protein ZNF185 antibody
    • P1 A antibody
    • P1-A antibody
    • Zinc finger protein 185 (LIM domain) antibody
    • Zinc finger protein 185 antibody
    • ZN185_HUMAN antibody
    • ZNF185 antibody
    see all

Images

  • Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human lung carcinoma  labeling ZNF185 with ab175603 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-ZNF185 antibody (ab175603) at 1/1000 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size: 74 kDa

References

ab175603 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175603.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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