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    znf217-antibody-ab117798.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZNF217 antibody (ab117798)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-ZNF217 antibody (ab117798)

    Key features and details

    • Rabbit polyclonal to ZNF217
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-ZNF217 antibody
      See all ZNF217 primary antibodies
    • Description

      Rabbit polyclonal to ZNF217
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide, corresponding to a region between amino acids 100-150 of Human ZNF217 (NP_006517.1).

    • Positive control

      • Jurkat whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris buffered saline

      pH 7-8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab117798 was affinity purified using an epitope specific to ZNF217 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Proto-oncogenes
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab117798 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at 2-10 µg/mg of lysate.
    Notes
    IP
    Use at 2-10 µg/mg of lysate.

    Target

    • Function

      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O75362 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7764 Human
      • Omim: 602967 Human
      • SwissProt: O75362 Human
      • Unigene: 155040 Human
      • Unigene: 711564 Human
      • Alternative names

        • ZABC 1 antibody
        • ZABC1 antibody
        • Zinc finger protein 217 antibody
        • ZN 217 antibody
        • ZN217 antibody
        • ZN217_HUMAN antibody
        • ZNF 217 antibody
        • ZNF217 antibody
        see all

      Images

      • Immunoprecipitation - Anti-ZNF217 antibody (ab117798)
        Immunoprecipitation - Anti-ZNF217 antibody (ab117798)
        Lane 1: Staining of ZNF217 in Jurkat whole cell lysate immunoprecipitated using ab117798 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 115 kDa. Exposure time: 30 seconds

      Protocols

      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab117798? Please let us know so that we can cite the reference in this datasheet.

      ab117798 has been referenced in 2 publications.

      • Pang B  et al. Landscape of tumor suppressor long noncoding RNAs in breast cancer. J Exp Clin Cancer Res 38:79 (2019). PubMed: 30764831
      • Zhu HY  et al. Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-ß2 by targeting ZNF217 via miR-200c in keloid fibroblasts. Sci Rep 6:24728 (2016). ChIP . PubMed: 27090737

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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