Overview

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB
  • Application notes

    This antibody gave a positive result in ELISA against the immunizing peptide (ab48177).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ZABC 1 antibody
      • ZABC1 antibody
      • Zinc finger protein 217 antibody
      • ZN 217 antibody
      • ZN217 antibody
      • ZN217_HUMAN antibody
      • ZNF 217 antibody
      • ZNF217 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Anti-ZNF217 antibody (ab48133) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 8 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 115 kDa
      Observed band size: 160 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Baran-Gale J  et al. An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells. RNA 22:1592-603 (2016). WB ; Human . Read more (PubMed: 27539783) »
    • Cohen PA  et al. The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value. Oncotarget 6:41566-81 (2015). Read more (PubMed: 26431164) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human embryonic stem cell lines)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    human embryonic stem cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Embryonic stem cell (HES2))
    Permeabilization
    Yes - 0.25% Trition X-100
    Specification
    Human Embryonic stem cell (HES2)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human embryonic stem cells)
    Specification
    human embryonic stem cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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