Recombinant
RabMAb

Recombinant Anti-ZNF217 antibody [EPR6362] (ab124927)

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Overview

  • Product name

    Anti-ZNF217 antibody [EPR6362]
    See all ZNF217 primary antibodies

  • Description

    Rabbit monoclonal [EPR6362] to ZNF217

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ZNF217 aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MCF7, PC3 and Caco2 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab124927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.

    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ZABC 1 antibody
        • ZABC1 antibody
        • Zinc finger protein 217 antibody
        • ZN 217 antibody
        • ZN217 antibody
        • ZN217_HUMAN antibody
        • ZNF 217 antibody
        • ZNF217 antibody
        see all

      Images

      • Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
        Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
        All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

        Lane 1 : MCF7 cell lysates
        Lane 2 : PC3 cell lysates
        Lane 3 : Caco2 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

        Predicted band size: 115 kDa

      • Other - Anti-ZNF217 antibody [EPR6362] (ab124927)
        Other - Anti-ZNF217 antibody [EPR6362] (ab124927)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab124927 has not yet been referenced specifically in any publications.

      Publishing research using ab124927? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-ZNF217 antibody [EPR6362]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (human ESCs)
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Loading amount
      30 µg
      Specification
      human ESCs
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 25 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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