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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-ZNF217 antibody [EPR6362] (ab124927)

  • Datasheet
  • SDS
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Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
  • OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
  • Anti-ZNF217 antibody [EPR6362] (ab124927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6362] to ZNF217
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-ZNF217 antibody [EPR6362]
    See all ZNF217 primary antibodies
  • Description

    Rabbit monoclonal [EPR6362] to ZNF217
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ZNF217 aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MCF7, PC3 and Caco2 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6362
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (ab240008)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O75362 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7764 Human
      • Omim: 602967 Human
      • SwissProt: O75362 Human
      • Unigene: 155040 Human
      • Unigene: 711564 Human
      • Alternative names

        • ZABC 1 antibody
        • ZABC1 antibody
        • Zinc finger protein 217 antibody
        • ZN 217 antibody
        • ZN217 antibody
        • ZN217_HUMAN antibody
        • ZNF 217 antibody
        • ZNF217 antibody
        see all

      Images

      • Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
        Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
        All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

        Lane 1 : MCF7 cell lysates
        Lane 2 : PC3 cell lysates
        Lane 3 : Caco2 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

        Predicted band size: 115 kDa

      • OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
        OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ZNF217 antibody [EPR6362] (ab124927)
        Anti-ZNF217 antibody [EPR6362] (ab124927)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab124927? Please let us know so that we can cite the reference in this datasheet.

      ab124927 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-ZNF217 antibody [EPR6362]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (human ESCs)
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Loading amount
      30 µg
      Specification
      human ESCs
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 25 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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