Name: Anti-ZNF217 antibody [EPR6362]
Brand: RabMAb
SKU: ab124927
Rating: 3 (1 reviews)

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Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6362] to ZNF217
Suitable for: WB
Reacts with: Human

Product name

Anti-ZNF217 antibody [EPR6362]
See all ZNF217 primary antibodies
Description

Rabbit monoclonal [EPR6362] to ZNF217
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Synthetic peptide within Human ZNF217 aa 1000-1100 (C terminal). The exact sequence is proprietary.
Positive control
- MCF7, PC3 and Caco2 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 9.30 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6362
Isotype

IgG
Research areas

Alternative Versions
- Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (ab240008)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).

Notes
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).

Application notes

Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Function

Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Cellular localization

Nucleus.
Target information above from: UniProt accession O75362 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7764 Human
- Omim: 602967 Human
- SwissProt: O75362 Human
- Unigene: 155040 Human
- Unigene: 711564 Human
Alternative names
- ZABC 1 antibody
- ZABC1 antibody
- Zinc finger protein 217 antibody
- ZN 217 antibody
- ZN217 antibody
- ZN217_HUMAN antibody
- ZNF 217 antibody
- ZNF217 antibody
see all

Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)

All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

Lane 1 : MCF7 cell lysates
Lane 2 : PC3 cell lysates
Lane 3 : Caco2 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 115 kDa
OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-ZNF217 antibody [EPR6362] (ab124927)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab124927? Please let us know so that we can cite the reference in this datasheet.

ab124927 has not yet been referenced specifically in any publications.

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Application

Western blot

Sample

Human Cell lysate - whole cell (human ESCs)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

30 µg

Specification

human ESCs

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

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Verified customer

Submitted Jan 25 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As

Western blot abreview for Anti-ZNF217 antibody [EPR6362]

Dissociation constant (K_D)