Overview

  • Product name

    Anti-ZNF224 antibody
  • Description

    Mouse polyclonal to ZNF224
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-707 of Human ZNF224 (AAH51902.1)

  • Positive control

    • ZNF224 transfected 293T cell line. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml. Antibody purification with Protein A will be needed prior to use.
WB 1/500 - 1/1000. Predicted molecular weight: 82 kDa.

Target

  • Function

    May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
  • Tissue specificity

    Ubiquitous. Mainly expressed in fetal tissues.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization

    Nucleus. Colocalizes with DEPDC1A at the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BMZF 2 antibody
    • BMZF-2 antibody
    • BMZF2 antibody
    • Bone marrow zinc finger 2 antibody
    • KOX22 antibody
    • Zinc finger 2, bone marrow antibody
    • Zinc finger protein 224 antibody
    • Zinc finger protein 233 antibody
    • Zinc finger protein 255 antibody
    • Zinc finger protein 27 (KOX 22) antibody
    • Zinc finger protein 27 antibody
    • Zinc finger protein KOX22 antibody
    • Zinc finger protein ZNF255 antibody
    • ZN224_HUMAN antibody
    • ZNF224 antibody
    • ZNF255 antibody
    • ZNF27 antibody
    see all

Images

  • All lanes : Anti-ZNF224 antibody (ab168669) at 1/500 dilution

    Lane 1 : ZNF224 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 82 kDa

  • Immunofluorescence analysis of HeLa cells labeling ZNF224; produced using the purified version of this antibody. For IHC and IF applications, antibody purification with Protein A will be needed prior to use.

References

This product has been referenced in:

  • Cho JG  et al. MED28 increases the colony-forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncol Lett 15:3147-3154 (2018). Read more (PubMed: 29435049) »
See 1 Publication for this product

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