Key features and details
- Mouse polyclonal to ZNF224
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF224 antibody
DescriptionMouse polyclonal to ZNF224
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- ZNF224 transfected 293T cell line. HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
Our Abpromise guarantee covers the use of ab168669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml. Antibody purification with Protein A will be needed prior to use.|
|WB||1/500 - 1/1000. Predicted molecular weight: 82 kDa.|
FunctionMay be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
Tissue specificityUbiquitous. Mainly expressed in fetal tissues.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 18 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Cellular localizationNucleus. Colocalizes with DEPDC1A at the nucleus.
- Information by UniProt
- BMZF 2 antibody
- BMZF-2 antibody
- BMZF2 antibody
All lanes : Anti-ZNF224 antibody (ab168669) at 1/500 dilution
Lane 1 : ZNF224 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 82 kDa
Immunofluorescence analysis of HeLa cells labeling ZNF224; produced using the purified version of this antibody. For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
ab168669 has been referenced in 1 publication.
- Cho JG et al. MED28 increases the colony-forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncol Lett 15:3147-3154 (2018). PubMed: 29435049