Key features and details
- Rabbit polyclonal to ZNF24
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF24 antibody
See all ZNF24 primary antibodies
DescriptionRabbit polyclonal to ZNF24
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176589 was affinity purified using an epitope specific to ZNF24 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscription factor required for myelination of differentiated oligodendrocytes. Required for the conversion of oligodendrocytes from the premyelinating to the myelinating state. In the developing central nervous system (CNS), involved in the maintenance in the progenitor stage by promoting the cell cycle. Specifically binds to the 5'-TCAT-3' DNA sequence (By similarity). Has transcription repressor activity in vitro.
Tissue specificityExpressed in many tissues except in heart.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
- Information by UniProt
- KOX17 antibody
- Retinoic acid suppression protein A antibody
- RSG A antibody
Detection of ZNF24 by Western Blot of Immunprecipitate.
ab176589 at 0.4µg/ml labeling ZNF24 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded) immunoprecipitated using ab176589 at 6µg/mg lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes : Anti-ZNF24 antibody (ab176589) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 3 minutes
ab176589 has not yet been referenced specifically in any publications.