Recombinant Anti-ZNF24 antibody [EPR13975] (ab180121)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13975] to ZNF24
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ZNF24 antibody [EPR13975]
See all ZNF24 primary antibodies -
Description
Rabbit monoclonal [EPR13975] to ZNF24 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ZNF24 aa 100-200. The exact sequence is proprietary.
Database link: P17028 -
Positive control
- HeLa cells; Jurkat, HeLa and 293 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13975 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
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Notes |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Transcription factor required for myelination of differentiated oligodendrocytes. Required for the conversion of oligodendrocytes from the premyelinating to the myelinating state. In the developing central nervous system (CNS), involved in the maintenance in the progenitor stage by promoting the cell cycle. Specifically binds to the 5'-TCAT-3' DNA sequence (By similarity). Has transcription repressor activity in vitro. -
Tissue specificity
Expressed in many tissues except in heart. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7572 Human
- Entrez Gene: 59057 Mouse
- Entrez Gene: 360204 Rat
- Omim: 194534 Human
- SwissProt: P17028 Human
- SwissProt: Q91VN1 Mouse
- SwissProt: Q7TNK3 Rat
- Unigene: 514802 Human
see all -
Alternative names
- KOX17 antibody
- Retinoic acid suppression protein A antibody
- RSG A antibody
see all
Images
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Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ZNF24with ab180121 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
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All lanes : Anti-ZNF24 antibody [EPR13975] (ab180121) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab180121 has been referenced in 1 publication.
- Le Z et al. Predictive single nucleotide polymorphism markers for acute oral mucositis in patients with nasopharyngeal carcinoma treated with radiotherapy. Oncotarget 8:63026-63037 (2017). WB . PubMed: 28968968