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    znf24-antibody-epr13975-ab180121.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-ZNF24 antibody [EPR13975] (ab180121)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Flow Cytometry - Anti-ZNF24 antibody [EPR13975] (ab180121)
  • Western blot - Anti-ZNF24 antibody [EPR13975] (ab180121)
  • Anti-ZNF24 antibody [EPR13975] (ab180121)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13975] to ZNF24
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ZNF24 antibody [EPR13975]
    See all ZNF24 primary antibodies
  • Description

    Rabbit monoclonal [EPR13975] to ZNF24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ZNF24 aa 100-200. The exact sequence is proprietary.
    Database link: P17028

  • Positive control

    • HeLa cells; Jurkat, HeLa and 293 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13975
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab180121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcription factor required for myelination of differentiated oligodendrocytes. Required for the conversion of oligodendrocytes from the premyelinating to the myelinating state. In the developing central nervous system (CNS), involved in the maintenance in the progenitor stage by promoting the cell cycle. Specifically binds to the 5'-TCAT-3' DNA sequence (By similarity). Has transcription repressor activity in vitro.
  • Tissue specificity

    Expressed in many tissues except in heart.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17028 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7572 Human
    • Entrez Gene: 59057 Mouse
    • Entrez Gene: 360204 Rat
    • Omim: 194534 Human
    • SwissProt: P17028 Human
    • SwissProt: Q91VN1 Mouse
    • SwissProt: Q7TNK3 Rat
    • Unigene: 514802 Human
    • Unigene: 417427 Mouse
    • Unigene: 162286 Rat
    see all
  • Alternative names

    • KOX17 antibody
    • Retinoic acid suppression protein A antibody
    • RSG A antibody
    • RSG-A antibody
    • RSGA antibody
    • Zfp191 antibody
    • Zinc finger and SCAN domain containing protein 3 antibody
    • Zinc finger and SCAN domain-containing protein 3 antibody
    • Zinc finger protein 191 antibody
    • Zinc finger protein 24 antibody
    • Zinc finger protein KOX17 antibody
    • ZNF 24 antibody
    • ZNF191 antibody
    • ZNF24 antibody
    • ZNF24_HUMAN antibody
    • ZSCAN 3 antibody
    • ZSCAN3 antibody
    see all

Images

  • Flow Cytometry - Anti-ZNF24 antibody [EPR13975] (ab180121)
    Flow Cytometry - Anti-ZNF24 antibody [EPR13975] (ab180121)

    Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ZNF24 with ab180121 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.

  • Western blot - Anti-ZNF24 antibody [EPR13975] (ab180121)
    Western blot - Anti-ZNF24 antibody [EPR13975] (ab180121)
    All lanes : Anti-ZNF24 antibody [EPR13975] (ab180121) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Anti-ZNF24 antibody [EPR13975] (ab180121)
    Anti-ZNF24 antibody [EPR13975] (ab180121)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab180121? Please let us know so that we can cite the reference in this datasheet.

    ab180121 has been referenced in 1 publication.

    • Le Z  et al. Predictive single nucleotide polymorphism markers for acute oral mucositis in patients with nasopharyngeal carcinoma treated with radiotherapy. Oncotarget 8:63026-63037 (2017). WB . PubMed: 28968968

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab180121.
    Please use the links above to contact us or submit feedback about this product.

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