Overview

  • Product name
    Anti-ZNF256 antibody [EPR11637]
  • Description
    Rabbit monoclonal [EPR11637] to ZNF256
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ZNF256 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y2P7

  • Positive control
    • K562, HeLa and MCF7 cell lysates, HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11637
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 15 C2H2-type zinc fingers.
      Contains 1 KRAB domain.
    • Domain
      The KRAB domain mediates interaction with TRIM28 and is required for transcriptional repressor activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • anti BMZF3 antibody
      • BMZF 3 antibody
      • BMZF-3 antibody
      • BMZF3 antibody antibody
      • Bone marrow zinc finger 3 antibody
      • Zinc finger protein 256 antibody
      • ZN256_HUMAN antibody
      • ZNF256 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling ZNF256, using ab170906 at 1/10 dilution.

    • Immunofluorescence analysis of HeLa cells, labeling ZNF256 using ab170906 at a 1/50 dilution.

    • All lanes : Anti-ZNF256 antibody [EPR11637] (ab170906) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 72 kDa

    References

    ab170906 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170906.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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