Key features and details
- Rabbit polyclonal to ZNF300
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF300 antibody
DescriptionRabbit polyclonal to ZNF300
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Rat brain and Mouse intestine tissue lysates; Rat brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
|WB||1/100 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 69 kDa).|
FunctionHas a transcriptional repressor activity.
Tissue specificityExpressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Information by UniProt
- DKFZp686B24204 antibody
- Kruppel like zinc finger protein antibody
- OTTHUMP00000160554 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat brain tissue labeling ZNF300 with ab202965 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-ZNF300 antibody (ab202965) at 1/200 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Mouse intestine tissue lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 69 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
ab202965 has not yet been referenced specifically in any publications.