Anti-ZNF312/FEZF2 antibody (ab69436)
Key features and details
- Rabbit polyclonal to ZNF312/FEZF2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-ZNF312/FEZF2 antibody
See all ZNF312/FEZF2 primary antibodies -
Description
Rabbit polyclonal to ZNF312/FEZF2 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spinal cord tissue lysate. ICC/IF: Neuro2a cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Relevance
ZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 55079 Human
- Entrez Gene: 54713 Mouse
- Entrez Gene: 305719 Rat
- Omim: 607414 Human
- SwissProt: Q8TBJ5 Human
- SwissProt: Q9ESP5 Mouse
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Alternative names
- Fez antibody
- FEZ family zinc finger protein 2 antibody
- FEZF 2 antibody
see all
Images
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ab69436 staining Fezf2 in Neuro2a cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab69436 at 1/1000 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). The secondary antibody (shown in green) was ab150081 Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 17 kDa, 33 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab69436 has been referenced in 4 publications.
- Paramo B et al. Neuregulin-4 Is Required for Maintaining Soma Size of Pyramidal Neurons in the Motor Cortex. eNeuro 8:N/A (2021). PubMed: 33495243
- Chen Z et al. Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-?B signaling pathway. Lab Invest 98:1225-1236 (2018). PubMed: 29925938
- Qu C et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem 291:7661-8 (2016). PubMed: 26861874
- Shu XS et al. FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma. Carcinogenesis 34:1984-93 (2013). WB . PubMed: 23677067