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    znf312fezf2-antibody-ab69436.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZNF312/FEZF2 antibody (ab69436)

  • Datasheet
Reviews (4)Q&A (6)References (3)

Product price, shipping and contact information

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Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)

Key features and details

  • Rabbit polyclonal to ZNF312/FEZF2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ZNF312/FEZF2 antibody
    See all ZNF312/FEZF2 primary antibodies
  • Description

    Rabbit polyclonal to ZNF312/FEZF2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse ZNF312/FEZF2 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101386)

  • Positive control

    • This antibody gave a positive signal in Mouse spinal cord tissue lysate.
  • General notes

     This product was previously labelled as ZNF312

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
WB
Mouse
All applications
Cow
Human
Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 µg/ml.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • Relevance

    ZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 55079 Human
    • Entrez Gene: 54713 Mouse
    • Entrez Gene: 305719 Rat
    • Omim: 607414 Human
    • SwissProt: Q8TBJ5 Human
    • SwissProt: Q9ESP5 Mouse
    • Unigene: 241523 Human
    • Alternative names

      • Fez antibody
      • FEZ family zinc finger protein 2 antibody
      • FEZF 2 antibody
      • FEZF2 antibody
      • FEZL antibody
      • Forebrain embryonic zinc finger like protein 2 antibody
      • TOF antibody
      • ZFP 312 antibody
      • ZFP312 antibody
      • Zinc finger FEZL antibody
      • Zinc finger protein 312 antibody
      • Zinc finger protein FEZ like antibody
      • ZNF 312 antibody
      see all

    Images

    • Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)
      Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)
      Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 48 kDa
      Additional bands at: 17 kDa, 33 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)
      Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)
      ICC/IF image of ab69436 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab69436? Please let us know so that we can cite the reference in this datasheet.

    ab69436 has been referenced in 3 publications.

    • Chen Z  et al. Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-?B signaling pathway. Lab Invest 98:1225-1236 (2018). PubMed: 29925938
    • Qu C  et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem 291:7661-8 (2016). PubMed: 26861874
    • Shu XS  et al. FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma. Carcinogenesis 34:1984-93 (2013). WB . PubMed: 23677067

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ZNF312/FEZF2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSC derived cortical neurons)
    Permeabilization
    Yes - 0.1% TritonX
    Specification
    iPSC derived cortical neurons
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Read More

    Rebecca Chowdhury

    Verified customer

    Submitted Feb 28 2018

    Western blot abreview for Anti-ZNF312/FEZF2 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Induced Pluripotent Stem cell derived glutamatergi)
    Gel Running Conditions
    Reduced Denaturing (Bolt 4-12% Bis-Tris Plus Gels)
    Loading amount
    4 µg
    Specification
    Induced Pluripotent Stem cell derived glutamatergi
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Erika Yeh

    Verified customer

    Submitted Jun 26 2015

    Immunocytochemistry abreview for Anti-ZNF312 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Induced Pluripotent Stem cell derived neurons)
    Permeabilization
    Yes - 90% Methanol
    Specification
    Induced Pluripotent Stem cell derived neurons
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Erika Yeh

    Verified customer

    Submitted Jun 19 2015

    Question

    We didn't have a chance to use the code before the expiration date. Just wondering if it's possible to extend it?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2012

    Answer


    I would be happy to offer you another code since you weren't able to use the original code. Our AbTrial program has changed though, so the code is worth the price you paid for the antibody, in this case $337. You use the code in the same way though.

    This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2012

    Question

    I'm using ab69436 in WB and seeing multiple bands so I can't determine the band of interest.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab36788.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Oct 23 2012

    Question

    ReviewID: xxx AbID: 69436, Anti-ZNF312 antibody Rating: Inconclusive Sample: Species: Mouse Type: Tissue sections Specification: brain Application: Application: Fixative: 70% Ethanol Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: pH6 or high pH citrate buffer Permeabilization: Yes Blocking step: Superblock (other company) as blocking agent for 15 mins at 25°C Other detail: Dilution: 1/100 Incubation time: 16 hours at 4°C Diluent: 1:10 blocking buffer in PBS Secondary Antibody: Name: Non-Abcam Antibody was used: Alexa Fluor® 488 Dye (other company) Conjugation: Alexa Fluor® 488 Dilution: 1/1000 Additional Data: Additional Notes: Tried with or without antigen retrieval (high or low pH citrate buffer). Expected to see pyramidal neuronal staining in layer V, but it was either in all neurons (weak) or no staining at all.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 02 2012

    Answer

    Thank you for submitting an Abreview for ab69436. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

    - If you are fixing with 70% ethanol only, an antigen retrieval step is not necessary. Only PFA or formalin fixation requires antigen retrieval.
    - Also, if your sections are thin enough (15 um or less), a permeabilization step is not necessary either as the tissue cells are being cut (average cell diameter is 100 um).

    If you decide to follow these suggestions, please let me know if they are helpful.
    I wish you good luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Oct 02 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ZNF312 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    70% Ethanol
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 or high pH citrate buffer
    Permeabilization
    Yes - blocking buffer containing 0.3% Triton X-100
    Blocking step
    Superblock (thermo) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 01 2012

    Question

    I would like to proceed the test. I am a grad student *

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2012

    Answer

    DISCOUNT CODE: *
    Expiration date: *

    I am very pleased to hear you would like to accept our offer and test ab69436 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2012

    Question

    I would like to know if the following conditions will qualify the testing discount.

    product: anti-ZNF312 (ab69436)

    Application: IF/IHC on mouse brain paraffin sections

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2012

    Answer

    Thank you very much for your interest in ab69436.

    To our knowledge, ab69436 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab69436 now, test it inIHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps and reply with the name of the institution that you are affiliated with:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab69436 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab69436 so please wait for my reply before ordering.

    2. Purchase ab69436 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-P.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab69436 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2012

    Question

    Problems with antibodies in IHC-FrFl mouse brain sections

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2012

    Answer

    Thank you for contacting Abcam earlier today.

    I am sorry about the issues you have been having with ab18465 in IHC-FrFl. as we discussed, I am sending you a new vial of this antibody, from a different lot to test out.

    As for ab69436, as we had not tested it in IHC-FrFl, we could not guarantee that it would work for you in this application, but if you can send me a copy of the protocol that you are using than I would be happy to see if there is anything I can suggest.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Jun 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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