Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF316
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ZNF316 aa 475-525. The exact sequence is proprietary.
    Sequence:

    HQAVHTADRPHCCPDCGQAFRLRADFQRHRRGGGCAEAGGDGPRREPGET A


    Database link: A6NFI3

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. Contains 1 KRAB domain.
  • Cellular localization
    Nucleus
  • Database links
  • Alternative names
    • Zinc finger protein 316 antibody
    • ZNF316 antibody

Images

  • All lanes : Anti-ZNF316 antibody (ab176708) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa


    Exposure time: 30 seconds
  • ab176708 at 0.4 µg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.

    Lane 1: ab176708 at 6 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab176708 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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