Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 108 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceMay be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. Contains 1 KRAB domain.
- Zinc finger protein 316 antibody
- ZNF316 antibody
All lanes : Anti-ZNF316 antibody (ab176708) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds
ab176708 at 0.4 µg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176708 at 6 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab176708 has not yet been referenced specifically in any publications.