Key features and details
- Rabbit polyclonal to ZNF324
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF324 antibody
See all ZNF324 primary antibodies
DescriptionRabbit polyclonal to ZNF324
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ZNF324 aa 1-270.
MAFEDVAVYFSQEEWGLLDTAQRALYRRVMLDNFALVASLGLSTSRPRVV IQLERGEEPWVPSGTDTTLSRTTYRRRNPGSWSLTEDRDVSGEWPRAFPD TPPGMTTSVFPVAGACHSVKSLQRQRGASPSRERKPTGVSVIYWERLLLG SGSGQASVSLRLTSPLRPPEGVRLREKTLTEHALLGRQPRTPERQKPCAQ EVPGRTFGSAQDLEAAGGRGHHRMGAVWQEPHRLLGGQEPSTWDELGEAL HAGEKSFECRACSKVFVKSS
Database link: O75467
- IHC-P: Human colon cancer and gastric cancer tissues. WB: A549, HEK-293T, MCF7 and Jurkat whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 9 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Information by UniProt
- Z324A_HUMAN antibody
- ZF5128 antibody
- Zinc finger protein 324A antibody
All lanes : Anti-ZNF324 antibody (ab225914) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Paraffin-embedded human colon cancer tissue stained for ZNF324 using ab225914 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for ZNF324 using ab225914 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225914 has not yet been referenced specifically in any publications.