Overview

  • Product name

    Anti-ZNF365 antibody [EPR10216]
    See all ZNF365 primary antibodies
  • Description

    Rabbit monoclonal [EPR10216] to ZNF365
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ZNF365 (Uniprot: Q70YC4).

  • Positive control

    • Human fetal kidney, Jurkat and HepG2 cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10216
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • All lanes : Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 24 kDa

    • Immunofluorescent analysis of Jurkat cells labeling ZNF365 with ab157459 at 1/50 dilution.
    • Flow cytometic analysis of permeabilized Jurkat cells labeling ZNF365 with ab157459 at 1/10 dilution (red) or a rabbit IgG negative (green).
    • Detection of ZNF365 by Western Blot of Immunprecipitate. Jurkat cell lysate immunoprecipitated using ab157459 at 1/10 dilution.

      Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution + Immunoprecipitation pellet from Jurkat cell lysate at 10 µg

    References

    ab157459 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157459.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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