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    znf423-antibody-ab169096.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZNF423 antibody (ab169096)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-ZNF423 antibody (ab169096)
  • Immunoprecipitation - Anti-ZNF423 antibody (ab169096)

Key features and details

  • Rabbit polyclonal to ZNF423
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ZNF423 antibody
    See all ZNF423 primary antibodies
  • Description

    Rabbit polyclonal to ZNF423
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 250-300 of Human ZNF423 (NP_055884.2, Uniprot: Q2M1K9)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Retinoic & Retinoid
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Neuroscience
    • Sensory System
    • Olfactory system
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Elephant
Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
  • Tissue specificity

    Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • Domain

    Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q2M1K9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23090 Human
    • Omim: 604557 Human
    • SwissProt: Q2M1K9 Human
    • Unigene: 530930 Human
    • Alternative names

      • Early B cell factor associated zinc finger protein antibody
      • Ebfaz antibody
      • hOAZ antibody
      • JBTS19 antibody
      • KIAA0760 antibody
      • NPHP14 antibody
      • Nur12 antibody
      • OAZ antibody
      • OLF 1/EBF associated zinc finger antibody
      • OLF1/EBF associated zinc finger protein antibody
      • Olf1/EBF-associated zinc finger protein antibody
      • Roaz antibody
      • Smad and Olf interacting zinc finger protein antibody
      • Smad- and Olf-interacting zinc finger protein antibody
      • Zfp104 antibody
      • ZFP423 antibody
      • zinc finger protein 423, mouse, homolog of antibody
      • Zinc finger protein 423 antibody
      • ZN423_HUMAN antibody
      • ZNF423 antibody
      • ZNF423 zinc finger protein 423 antibody
      see all

    Images

    • Western blot - Anti-ZNF423 antibody (ab169096)
      Western blot - Anti-ZNF423 antibody (ab169096)
      All lanes : Anti-ZNF423 antibody (ab169096) at 0.4 µg/ml

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 145 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-ZNF423 antibody (ab169096)
      Immunoprecipitation - Anti-ZNF423 antibody (ab169096)
      Immunoprecipitation analysis of 1 mg (20% of IP loaded) of 293T whole cell lysate using: 1) ab169096 (6 µg/mg lysate), 2) another rabbit anti-ZNF423 antibody and 3) control IgG. For blotting ab169096 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 3 minutes.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab169096? Please let us know so that we can cite the reference in this datasheet.

    ab169096 has been referenced in 3 publications.

    • Xin W  et al. BCAT1 binds the RNA-binding protein ZNF423 to activate autophagy via the IRE1-XBP-1-RIDD axis in hypoxic PASMCs. Cell Death Dis 11:764 (2020). PubMed: 32938905
    • Wang G  et al. 4-Hydroxytamoxifen enhances sensitivity of estrogen receptor a-positive breast cancer to docetaxel in an estrogen and ZNF423 SNP-dependent fashion. Breast Cancer Res Treat 175:567-578 (2019). PubMed: 30937657
    • Qi YY  et al. A Rare Variant (rs933717) at FBXO31-MAP1LC3B in Chinese Is Associated With Systemic Lupus Erythematosus. Arthritis Rheumatol 70:287-297 (2018). PubMed: 29044928

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