Anti-ZNF423 antibody (ab169096)
Key features and details
- Rabbit polyclonal to ZNF423
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF423 antibody
See all ZNF423 primary antibodies -
Description
Rabbit polyclonal to ZNF423 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 250-300 of Human ZNF423 (NP_055884.2, Uniprot: Q2M1K9)
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Positive control
- 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
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| WB |
Human
|
| All applications |
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Elephant
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 145 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation. -
Tissue specificity
Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 30 C2H2-type zinc fingers. -
Domain
Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23090 Human
- Omim: 604557 Human
- SwissProt: Q2M1K9 Human
- Unigene: 530930 Human
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Alternative names
- Early B cell factor associated zinc finger protein antibody
- Ebfaz antibody
- hOAZ antibody
see all
Images
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Western blot - Anti-ZNF423 antibody (ab169096)All lanes : Anti-ZNF423 antibody (ab169096) at 0.4 µg/ml
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZNF423 antibody (ab169096)Immunoprecipitation analysis of 1 mg (20% of IP loaded) of 293T whole cell lysate using: 1) ab169096 (6 µg/mg lysate), 2) another rabbit anti-ZNF423 antibody and 3) control IgG. For blotting ab169096 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab169096 has been referenced in 3 publications.
- Xin W et al. BCAT1 binds the RNA-binding protein ZNF423 to activate autophagy via the IRE1-XBP-1-RIDD axis in hypoxic PASMCs. Cell Death Dis 11:764 (2020). PubMed: 32938905
- Wang G et al. 4-Hydroxytamoxifen enhances sensitivity of estrogen receptor a-positive breast cancer to docetaxel in an estrogen and ZNF423 SNP-dependent fashion. Breast Cancer Res Treat 175:567-578 (2019). PubMed: 30937657
- Qi YY et al. A Rare Variant (rs933717) at FBXO31-MAP1LC3B in Chinese Is Associated With Systemic Lupus Erythematosus. Arthritis Rheumatol 70:287-297 (2018). PubMed: 29044928