Anti-ZNF433 antibody (ab221993)
Key features and details
- Rabbit polyclonal to ZNF433
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF433 antibody -
Description
Rabbit polyclonal to ZNF433 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ZNF433 aa 653-672.
Sequence:KVFRCSSQLQVHGRAHCIDT
Database link: Q8N7K0 -
Positive control
- IHC-P: Human testis tissue. ICC/IF: RT4 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab221993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 19 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 163059 Human
- SwissProt: Q8N7K0 Human
- Unigene: 667776 Human
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Alternative names
- Zinc finger protein 433 antibody
- ZN433_HUMAN antibody
- ZNF433 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF433 antibody (ab221993)Paraffin-embedded human testis tissue stained for ZNF433 using ab221993 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF433 antibody (ab221993)PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for ZNF433 (green) using ab221993 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab221993 has not yet been referenced specifically in any publications.
