Anti-ZNF433 antibody (ab221993)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ZNF433 antibody
See all ZNF433 primary antibodies -
Description
Rabbit polyclonal to ZNF433 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ZNF433 aa 653-672.
Sequence:KVFRCSSQLQVHGRAHCIDT
Database link: Q8N7K0 -
Positive control
- IHC-P: Human testis tissue. ICC/IF: RT4 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab221993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 19 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 163059 Human
- SwissProt: Q8N7K0 Human
- Unigene: 667776 Human
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Alternative names
- Zinc finger protein 433 antibody
- ZN433_HUMAN antibody
- ZNF433 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF433 antibody (ab221993)Paraffin-embedded human testis tissue stained for ZNF433 using ab221993 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF433 antibody (ab221993)PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for ZNF433 (green) using ab221993 at 4 µg/ml in ICC/IF.
Datasheets and documents
References
ab221993 has not yet been referenced specifically in any publications.
