Key features and details
- Rabbit polyclonal to ZNF444
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF444 antibody
See all ZNF444 primary antibodies
DescriptionRabbit polyclonal to ZNF444
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 200-250 of Human ZNF44 (NP_060807.2).
- HeLa, Jurkat and 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117765 was affinity purified using an epitope specific to ZNF444 immobilized on solid support.
Our Abpromise guarantee covers the use of ab117765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 35 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
- Information by UniProt
- Endothelial zinc finger protein 2 antibody
- EZF 2 antibody
- EZF-2 antibody
All lanes : Anti-ZNF444 antibody (ab117765) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 30 seconds
Detection of ZNF444 by Western Blot of Immunprecipitate.
ab117765 at 1µg/ml staining ZNF444 in HeLa whole cell lysate immunoprecipitated using ab117765 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab117765 has not yet been referenced specifically in any publications.