Product nameAnti-ZNF444 antibody
See all ZNF444 primary antibodies
DescriptionRabbit polyclonal to ZNF444
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human ZNF444 according to NP_060807.2.
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab117812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 35 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
- Information by UniProt
- Endothelial zinc finger protein 2 antibody
- EZF 2 antibody
- EZF-2 antibody
All lanes : Anti-ZNF444 antibody (ab117812) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 30 seconds
ab117812 at 1 µg/ml staining ZNF444 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117812 at 6 µg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
ab117812 has not yet been referenced specifically in any publications.