Key features and details
- Mouse monoclonal [OTI4C6] to ZNF449
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ZNF449 antibody [OTI4C6]
DescriptionMouse monoclonal [OTI4C6] to ZNF449
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Recombinant full length protein corresponding to Human ZNF449 aa 1-518. (NP_689908) produced in E.coli.
MAVALGCAIQASLNQGSVFQEYDTDCEVFRQRFRQFQYREAAGPHEAFNK LWELCCQWLKPKMRSKEQILELLVLEQFLTILPTEIETWVREHCPENRER VVSLIEDLQRELEIPEQQVDMHDMLLEELAPVGTAHIPPTMHLESPALQV MGPAQEAPVAEAWIPQAGPPELNYGATGECQNFLDPGYPLPKLDMNFSLE NREEPWVKELQDSKEMKQLLDSKIGFEIGIENEEDTSKQKKMETMYPFIV TLEGNALQGPILQKDYVQLENQWETPPEDLQTDLAKLVDQQNPTLGETPE NSNLEEPLNPKPHKKKSPGEKPHRCPQCGKCFARKSQLTGHQRIHSGEEP HKCPECGKRFLRSSDLYRHQRLHTGERPYECTVCKKRFTRRSHLIGHQRT HSEEETYKCLECGKSFCHGSSLKRHLKTHTGEKPHRCHNCGKSFSRLTAL TLHQRTHTEERPFKCNYCGKSFRQRPSLVIHLRIHTGEKPYKCTHCSKSF RQRAGLIMHQVTHFRGLI
Database link: Q6P9G9
- WB: pCMV6-ENTRY ZNF449 transfected HEK-293T cell lysate. IHC-P: Human kidney carcinoma, lung carcinoma and bladder tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236427 in the following tested applications.
|WB||1/2000. Predicted molecular weight: 60 kDa.|
Heat-induced epitope retrieval by Tris-EDTA, pH 8.0.
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
- Information by UniProt
- FLJ23614 antibody
- OTTHUMP00000024072 antibody
- Zinc finger and SCAN domain containing protein 19 antibody
All lanes : Anti-ZNF449 antibody [OTI4C6] (ab236427) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ZNF449 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa
Paraffin-embedded human kidney carcinoma tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lung carcinoma tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.
ab236427 has not yet been referenced specifically in any publications.