Overview

  • Product name

    Anti-ZNF45 antibody
  • Description

    Rabbit polyclonal to ZNF45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human ZNF45 (internal sequence). The exact sequence is proprietary.
    Database link: Q02386

  • Positive control

    • IHC-P: Human colon carcinoma tissue. ICC/IF: A375 and HCT 116 cells. WB: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    ZNF45 belongs to the krueppel C2H2 type zinc finger protein family. It contains 18 C2H2 type zinc fingers and 1 KRAB domain. ZNF45 may be involved in transcriptional regulation.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • BRC1744 antibody
    • KOX5 antibody
    • Zinc finger protein 13 antibody
    • Zinc finger protein 45 (a Kruppel associated box (KRAB) domain polypeptide) antibody
    • Zinc finger protein 45 antibody
    • Zinc finger protein KOX5 antibody
    • ZNF13 antibody
    see all

Images

  • Paraffin-embedded human colon carcinoma tissue stained for ZNF45 with ab228574 at 1/250 dilution in immunohistochemical analysis.

  • Anti-ZNF45 antibody (ab228574) at 1/2000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 78 kDa



    7.5% SDS-PAGE

  • HCT 166 (human colorectal carcinoma cell line) cells stained for ZNF45 (green) using ab228574 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

  • A375 (human malignant melanoma cell line) cells stained for ZNF45 (green) using ab228574 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

References

ab228574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228574.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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