Overview

  • Product name

  • Description

    Rabbit polyclonal to ZNF485
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with ZNF485
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: RHSSGLVEHQ RLHTGEKPYK CNECGKAFPR SSALKQHKKI HNKERAMKCS, corresponding to amino acids 392-441 of Human ZNF485

  • Positive control

    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    May be involved in transcriptional regulation.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Zinc finger protein 485 antibody
    • ZN485_HUMAN antibody
    • ZNF485 antibody

Images

  • Lane 2 : Anti-ZNF485 antibody (ab50559) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa
    Observed band size: 35,50 kDa
    why is the actual band size different from the predicted?

References

ab50559 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50559.
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