Key features and details
- Rabbit polyclonal to ZNF516
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF516 antibody
DescriptionRabbit polyclonal to ZNF516
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 610-714 of Human ZNF516.
- Human prostate tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 10 C2H2-type zinc fingers.
- Information by UniProt
- C330029B10Rik antibody
- D230016L03 antibody
- HsT287 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121486 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121486, at 1/60 dilution, staining ZNF516 in paraffin-embedded Human prostate tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.
ab121486 has been referenced in 1 publication.
- Ili C et al. Loss of ZNF516 protein expression is related with HR-HPV infection and cervical preneoplastic lesions. Arch Gynecol Obstet N/A:N/A (2018). PubMed: 30564931