Overview

  • Product name

    Anti-ZNF521 antibody - N-terminal
    See all ZNF521 primary antibodies
  • Description

    Rabbit polyclonal to ZNF521 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ZNF521 (N terminal). The exact sequence is proprietary. 18 amino acid peptide from near the amino terminus.
    Database link: Q96K83

  • Positive control

    • Human small intestine tissue, HeLa cells, HeLa cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab189956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 2.5 - 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 148 kDa.

Target

  • Function

    Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.
  • Tissue specificity

    Predominantly expressed in hematopoietic cells. Present in organs and tissues that contain stem and progenitor cells, myeloid and/or lymphoid: placenta, spleen, lymph nodes, thymus, bone marrow and fetal liver. Within the hematopoietic system, it is abundant in CD34(+) cells but undetectable in mature peripheral blood leukocytes, and its levels rapidly decrease during the differentiation of CD34(+) cells in response to hemopoietins.
  • Involvement in disease

    Note=A chromosomal aberration involving ZNF521 is found in acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with PAX5. The translocation generates the PAX5-ZNF521 oncogene consisting of the N-terminus part of PAX5 and the C-terminus part of ZNF521.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • Domain

    Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and hematopoietic system.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B930086A16Rik antibody
    • DKFZp564D0764 antibody
    • Early hematopoietic zinc finger antibody
    • Early hematopoietic zinc finger protein antibody
    • EHZF antibody
    • Evi3 antibody
    • LYST-interacting protein 3 antibody
    • MGC142182 antibody
    • MGC142208 antibody
    • Zfp521 antibody
    • Zinc finger protein 521 antibody
    • ZN521_HUMAN antibody
    • Znf521 antibody
    see all

Images

  • All lanes : Anti-ZNF521 antibody - N-terminal (ab189956) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide

    Predicted band size: 148 kDa

  • Immunocytochemistry analysis of HeLa cells labeling ZNF521 using ab189956 at 2.5 ug/ml

  • Immunfluorescence analysis of HeLa cells labeling ZNF521 using ab189956 at 20 ug/ml

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling ZNF521 using ab189956 at 5 ug/ml

References

ab189956 has not yet been referenced specifically in any publications.

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