Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
Concentration information loading...
PurityProtein G purified
Purification notesab53675 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab53675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 82 kDa.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 13 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Information by UniProt
- Zinc finger protein 544 antibody
- Zinc finger protein AF020591 antibody
- ZN544_HUMAN antibody
Staining of immunizing recombinant ZNF544 fragment using anti-ZNF544 antibody [3359C4a] (ab53675).
Predicted band size: 82 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab53675 has not yet been referenced specifically in any publications.