Anti-ZNF549 antibody (ab243588)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ZNF549 antibody
See all ZNF549 primary antibodies -
Description
Rabbit polyclonal to ZNF549 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human ZNF549 aa 153-229.
Sequence:QDSGEKHIRKEESSALLLNSCKIPLSDNLFPCKDVEKDFPTILGLLQHQT THSRQEYAHRSRETFQQRRYKCEQVFN
Database link: Q6P9A3 -
Positive control
- IHC-P: Human Urinary bladder tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab243588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
May be involved in transcriptional regulation. There are two named isoforms. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 256051 Human
- Entrez Gene: 100172366 Orangutan
- SwissProt: Q6P9A3 Human
- SwissProt: Q5RBQ3 Orangutan
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Alternative names
- Zinc finger protein 549 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF549 antibody (ab243588)Paraffin-embedded human urinary bladder tissue stained for ZNF549 using ab243588 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF549 antibody (ab243588)4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling ZNF549 (Green) using ab243588 at 4 µg/ml in ICC/IF.
Datasheets and documents
References
ab243588 has not yet been referenced specifically in any publications.
