Anti-ZNF558 antibody [OTI2C4] (ab236428)
Key features and details
- Mouse monoclonal [OTI2C4] to ZNF558
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ZNF558 antibody [OTI2C4] -
Description
Mouse monoclonal [OTI2C4] to ZNF558 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human ZNF558 aa 1 to the C-terminus. (NP_653294) produced in E.coli.
Database link: Q96NG5 -
Positive control
- IHC-P: Human kidney tissue. WB: pCMV6-ENTRY ZNF558 cDNA transfected HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2C4 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/150.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer, pH 8.5 at 120°C for 3 minutes. |
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WB |
1/2000. Predicted molecular weight: 46 kDa.
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Notes |
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IHC-P
1/150. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer, pH 8.5 at 120°C for 3 minutes. |
WB
1/2000. Predicted molecular weight: 46 kDa. |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 9 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 148156 Human
- SwissProt: Q96NG5 Human
- Unigene: 659797 Human
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Alternative names
- FLJ30932 antibody
- Zinc finger protein 558 antibody
- ZN558_HUMAN antibody
- ZNF558 antibody
Images
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Paraffin-embedded human kidney tissue stained for ZNF558 using ab236428 at 1/150 dilution in immunohistochemical analysis.
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All lanes : Anti-ZNF558 antibody [OTI2C4] (ab236428) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ZNF558 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 46 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236428 has not yet been referenced specifically in any publications.