Overview

  • Product name

    Anti-ZNF580 antibody
  • Description

    Rabbit polyclonal to ZNF580
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog
  • Immunogen

    A region within synthetic peptide: PPPPKAPPFP KAEGPSSTPS SAAGPRPPRL GRHLLIDANG VPYTYTVQLE, corresponding to amino acids 21-70 of Human ZNF580

  • Positive control

    • Jurkat cell lysate, Human stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • Anti-ZNF580 antibody (ab59015) at 1 µg/ml + Jurkat cell lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Diluted in 5% skim milk / PBS buffer; gel concentration 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ZNF580 with ab59015 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the fundic gland. Magnification: 400X.

References

This product has been referenced in:

  • Wei S  et al. Novel Zinc Finger Transcription Factor ZFP580 Facilitates All-Trans Retinoic Acid -Induced Vascular Smooth Muscle Cells Differentiation by Rara-Mediated PI3K/Akt and ERK Signaling. Cell Physiol Biochem 50:2390-2405 (2018). Read more (PubMed: 30423583) »
  • Mao SY  et al. The role of ZFP580, a novel zinc finger protein, in TGF-mediated cytoprotection against chemical hypoxia-induced apoptosis in H9c2 cardiac myocytes. Mol Med Rep 15:2154-2162 (2017). Read more (PubMed: 28259939) »
See all 2 Publications for this product

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