Overview

  • Product name

  • Description

    Rabbit polyclonal to ZNF589
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 39-183 of Human ZNF589 (Q86UQ0).

  • Positive control

    • RT4 and U-251 MG cell lysates; Human bone marrow tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in hematopoietic stem/progenitor cell differentiation. May play a role as a DNA binding-dependent transcriptional repressor.
  • Tissue specificity

    Isoform 2 is widely expressed. Isoform 3 is only expressed in CD34(+) cells.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Domain

    The KRAB domain mediates interaction with TRIM28.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KRAB zinc finger protein SZF1 1 antibody
    • Stem cell zinc finger protein 1 antibody
    • SZF1 antibody
    • Zinc finger protein 589 antibody
    • ZN589_HUMAN antibody
    • ZNF589 antibody
    • ZNP589 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei and cytoplasm. Recommended concentration of ab121173 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121173 at 1/100 dilution staining ZNF589 in Paraffin-emebedded Human bone marrow tissue by Immunohistochemistry.

References

ab121173 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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