Anti-ZNF622 antibody (ab174657)

Reviews (1) Submit a question

Overview

  • Product name
    Anti-ZNF622 antibody
    See all ZNF622 primary antibodies
  • Description
    Rabbit polyclonal to ZNF622
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ZNF622 aa 125-175. The exact sequence is proprietary. NP_219482.1
    Sequence:

    KDAMNAAIQQAIKAQPSMSPKKAPPAPAKEARNVVAVGTGGRGTHDRDPS E


    Database link: Q969S3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174657 was affinity purified using an epitope specific to ZNF622 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May behave as an activator of the bound transcription factor, MYBL2, and be involved in embryonic development.
  • Tissue specificity
    Expressed in lung, kidney, spleen, liver and brain with lowest expression in kidney.
  • Sequence similarities
    Contains 2 U1-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by MELK. The phosphorylation may redirect the protein to the nucleus.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • MGC108936 antibody
      • Zfp622 antibody
      • Zinc finger protein 622 antibody
      • Zinc finger-like protein 9 antibody
      • ZN622_HUMAN antibody
      • ZNF622 antibody
      • ZPR9 antibody
      see all

    Images

    • Western blot - Anti-ZNF622 antibody (ab174657)
      Western blot - Anti-ZNF622 antibody (ab174657)
      All lanes : Anti-ZNF622 antibody (ab174657) at 0.100000001490116 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 54 kDa


      Exposure time: 10 seconds
    • Immunoprecipitation - Anti-ZNF622 antibody (ab174657)
      Immunoprecipitation - Anti-ZNF622 antibody (ab174657)

      Detection of ZNF622 by Western Blot of Immunoprecipitate.

    References

    ab174657 has not yet been referenced specifically in any publications.

    Publishing research using ab174657? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-ZNF622 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4~20%)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up