Anti-ZNF703 antibody [CL0654] (ab188031)
Key features and details
- Mouse monoclonal [CL0654] to ZNF703
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ZNF703 antibody [CL0654]
See all ZNF703 primary antibodies -
Description
Mouse monoclonal [CL0654] to ZNF703 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human ZNF703 aa 150-250.
Database link: Q9H7S9 -
Positive control
- Human breast carcinoma, colorectal adenocarcinoma, stomach, small intestine, rectum, placenta and liver tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
CL0654 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 58 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May function as a transcriptional repressor. -
Sequence similarities
Belongs to the Elbow/Noc family.
Contains 1 C2H2-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 80139 Human
- Entrez Gene: 353310 Mouse
- Entrez Gene: 680717 Rat
- SwissProt: Q9H7S9 Human
- SwissProt: P0CL69 Mouse
- Unigene: 726062 Human
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Alternative names
- FLJ14299 antibody
- ZEPPO1 antibody
- Zinc finger elbow-related proline domain protein 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach (ventricle cancer) tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human rectum tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling ZNF703 using ab188031 at a 1/50 dilution.
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All lanes : Anti-ZNF703 antibody [CL0654] (ab188031) at 1/500 dilution
Lane 1 : Negative control (vector only transfected HEK293T cell lysate)
Lane 2 : ZNF703 over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293 cells)
Predicted band size: 58 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab188031 has been referenced in 2 publications.
- Xu G et al. LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703. Cancer Cell Int 20:136 (2020). PubMed: 32351330
- Wang Y et al. Long Non-Coding RNA H19 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Breast Cancer Cells by Targeting miR-491-5p/ZNF703 Axis. Cancer Manag Res 12:9247-9258 (2020). PubMed: 33061615