Key features and details
- Rabbit polyclonal to ZNF704
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF704 antibody
DescriptionRabbit polyclonal to ZNF704
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- Human small intestine tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 1 C2H2-type zinc finger.
- Information by UniProt
- Gig1 antibody
- Zinc finger protein 704 antibody
- ZN704_HUMAN antibody
- ZNF704 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121804 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lanes 2-3 : Anti-ZNF704 antibody (ab121804)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing ZNF704 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemical staining of paraffin embedded Human small intestine tissue using 1/50 ab121804.
ab121804 has not yet been referenced specifically in any publications.