Product nameAnti-ZNF75 antibody [3424C1a]
DescriptionMouse monoclonal [3424C1a] to ZNF75
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment comprising of 50-200 amino acids from the internal region of Human ZNF75 (NM_007131).
- Recombinant fragment of human ZNF75.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab76563 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab76563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 59 kDa. A band of 32 kDa was detected when the immunizing recombinant protein fragment was used.|
|Dot blot||Use at an assay dependent dilution.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain.
- Information by UniProt
- D8C6 antibody
- MGC126327 antibody
- OTTHUMP00000024073 antibody
Anti-ZNF75 antibody [3424C1a] (ab76563) at 2 µg/ml + immunizing recombinant protein
Predicted band size: 59 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab76563 has not yet been referenced specifically in any publications.