Key features and details
- Rabbit polyclonal to ZNF774
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNF774 antibody
DescriptionRabbit polyclonal to ZNF774
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human colon tissue; RT4 cell lysate and Human Plasma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Information by UniProt
- Zinc finger protein 774 antibody
- ZN774_HUMAN antibody
- ZNF774 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and cytoskeleton (intermediate filaments). Recommended concentration of ab122137 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122137, at 1/200 dilution, staining ZNF774 in Human colon tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
All lanes : Anti-ZNF774 antibody (ab122137) at 1/500 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
ab122137 has not yet been referenced specifically in any publications.