Anti-ZNFN1A2/HELIOS antibody (ab129434)
Key features and details
- Rabbit polyclonal to ZNFN1A2/HELIOS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNFN1A2/HELIOS antibody
See all ZNFN1A2/HELIOS primary antibodies -
Description
Rabbit polyclonal to ZNFN1A2/HELIOS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human ZNFN1A2/HELIOS aa 25-75.
Database link: NP_057344.2 -
Positive control
- Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab129434 was affinity purified using an epitope specific to ZNFN1A2/HELIOS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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IP |
Use a concentration of 2 - 10 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
IP
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
Associates with Ikaros at centromeric heterochromatin. -
Sequence similarities
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22807 Human
- Omim: 606234 Human
- SwissProt: Q9UKS7 Human
- Unigene: 604950 Human
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Alternative names
- ANF1A2 antibody
- HELIOS antibody
- IKAROS family zinc finger 2 antibody
see all
Images
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All lanes : Anti-ZNFN1A2/HELIOS antibody (ab129434) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 minutes -
Detection of ZNFN1A2/HELIOS by Western Blot of Immunprecipitate.
ab129434 at 0.4 µg/ml staining ZNFN1A2/HELIOS in Jurkat whole cell lysate immunoprecipitated using ab129434 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG.
Chemiluminescence with an exposure time of 10 seconds. 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129434 has not yet been referenced specifically in any publications.