Key features and details
- Rabbit polyclonal to ZNFN1A2/HELIOS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNFN1A2/HELIOS antibody
See all ZNFN1A2/HELIOS primary antibodies
DescriptionRabbit polyclonal to ZNFN1A2/HELIOS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
Synthetic peptide corresponding to Human ZNFN1A2/HELIOS aa 25-75.
Database link: NP_057344.2
- Jurkat whole cell lysate (ab7899).
This product was previously labelled as ZNFN1A2
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129434 was affinity purified using an epitope specific to ZNFN1A2/HELIOS immobilized on solid support.
Our Abpromise guarantee covers the use of ab129434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionAssociates with Ikaros at centromeric heterochromatin.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
- Information by UniProt
- ANF1A2 antibody
- HELIOS antibody
- IKAROS family zinc finger 2 antibody
All lanes : Anti-ZNFN1A2/HELIOS antibody (ab129434) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 minutes
Detection of ZNFN1A2/HELIOS by Western Blot of Immunprecipitate.
ab129434 at 0.4 µg/ml staining ZNFN1A2/HELIOS in Jurkat whole cell lysate immunoprecipitated using ab129434 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG.
Chemiluminescence with an exposure time of 10 seconds. 3 seconds.
ab129434 has not yet been referenced specifically in any publications.