Key features and details
- Rabbit polyclonal to ZNFN1A2/HELIOS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZNFN1A2/HELIOS antibody
See all ZNFN1A2/HELIOS primary antibodies
DescriptionRabbit polyclonal to ZNFN1A2/HELIOS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rhesus monkey
Recombinant fragment within Human ZNFN1A2/HELIOS (internal sequence). The exact sequence is proprietary.
Database link: Q9UKS7
- WB: Jurkat whole cell and nuclear extract lysate. IHC: Mouse prostate tissue. ICC/IF: HeLa cells.
This product was previously labelled as ZNFN1A2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionAssociates with Ikaros at centromeric heterochromatin.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
- Information by UniProt
- ANF1A2 antibody
- HELIOS antibody
- IKAROS family zinc finger 2 antibody
All lanes : Anti-ZNFN1A2/HELIOS antibody (ab227429) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Paraffin-embedded mouse prostate tissue stained for ZNFN1A2/HELIOS using ab227429 at 1/500 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNFN1A2/HELIOS (green) using ab227429 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes. Counterstained: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227429 has not yet been referenced specifically in any publications.