Product nameAnti-ZNFN1A2/HELIOS antibody
See all ZNFN1A2/HELIOS primary antibodies
DescriptionRabbit polyclonal to ZNFN1A2/HELIOS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rhesus monkey
Recombinant fragment within Human ZNFN1A2/HELIOS (internal sequence). The exact sequence is proprietary.
Database link: Q9UKS7
- WB: Jurkat whole cell and nuclear extract lysate. IHC: Mouse prostate tissue. ICC/IF: HeLa cells.
This product was previously labelled as ZNFN1A2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionAssociates with Ikaros at centromeric heterochromatin.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
- Information by UniProt
- ANF1A2 antibody
- HELIOS antibody
- IKAROS family zinc finger 2 antibody
All lanes : Anti-ZNFN1A2/HELIOS antibody (ab227429) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Paraffin-embedded mouse prostate tissue stained for ZNFN1A2/HELIOS using ab227429 at 1/500 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNFN1A2/HELIOS (green) using ab227429 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes. Counterstained: Hoechst 33342 (blue).
ab227429 has not yet been referenced specifically in any publications.