Overview

  • Product name

    Anti-ZNHIT1 antibody
  • Description

    Rabbit polyclonal to ZNHIT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-154 of Human ZNHIT1 (UniProt ID: O43257).

  • Positive control

    • MOLT-4 whole cell lysate; Raji cells; HeLa cells; SW480 xenograft tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    ZNHIT1, Zinc finger HIT domain containing protein 1, appears to play a role in p53-mediated apoptosis induction.
  • Database links

  • Alternative names

    • CG1I antibody
    • CGBP1 antibody
    • Cyclin G1 binding protein 1 antibody
    • p18 Hamlet antibody
    • Zinc finger HIT domain containing protein 1 antibody
    • Zinc finger protein subfamily 4A member 1 antibody
    • zinc finger, HIT type 1 antibody
    • ZNFN4A1 antibody
    see all

Images

  • Anti-ZNHIT1 antibody (ab151248) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg

    Predicted band size: 18 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ZNHIT1 with ab151248 at 1/100 dilution. Lower panel was merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft tissue labeling ZNHIT1 with ab151248 at 1/500 dilution.

References

ab151248 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151248.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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