Key features and details
- Rabbit polyclonal to ZNHIT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZNHIT1 antibody
DescriptionRabbit polyclonal to ZNHIT1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- MOLT-4 whole cell lysate; Raji cells; HeLa cells; SW480 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceZNHIT1, Zinc finger HIT domain containing protein 1, appears to play a role in p53-mediated apoptosis induction.
- CG1I antibody
- CGBP1 antibody
- Cyclin G1 binding protein 1 antibody
Anti-ZNHIT1 antibody (ab151248) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ZNHIT1 with ab151248 at 1/100 dilution. Lower panel was merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft tissue labeling ZNHIT1 with ab151248 at 1/500 dilution.
ab151248 has not yet been referenced specifically in any publications.