Overview

  • Product name

    Anti-ZNRF1 antibody
  • Description

    Goat polyclonal to ZNRF1
  • Host species

    Goat
  • Specificity

    ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Mouse ZNRF1 aa 93-106 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Sequence:

    SDSTYAHGNGYQET


    Database link: Q91V17

  • Positive control

    • Znrf1 transfected N2a cells, Mouse embryo and adult brain lysates and Mouse cerebral cortex tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to substrates. May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin-protein ligase activity.
  • Tissue specificity

    Expressed primarily in the nervous system, with expression higher in developing brain relative to adult. Expressed at low levels in testis and thymus.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is required for E3 ligase activity.
  • Cellular localization

    Endosome. Lysosome. Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Associated with synaptic vesicle membranes in neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • B830022L21Rik antibody
    • DKFZp434E229 antibody
    • E3 ubiquitin protein ligase ZNRF1 antibody
    • E3 ubiquitin-protein ligase ZNRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ14846 antibody
    • MGC101991 antibody
    • MGC15430 antibody
    • Nerve injury gene 283 antibody
    • Nerve injury induced gene 283 protein antibody
    • Nerve injury-induced gene 283 protein antibody
    • NIN283 antibody
    • Rnf42 antibody
    • Zinc and ring finger 1 antibody
    • zinc and ring finger protein 1 antibody
    • zinc finger and ring finger protein 1 antibody
    • Zinc/RING finger protein 1 antibody
    • ZNRF 1 antibody
    • znrf1 antibody
    • ZNRF1_HUMAN antibody
    • Zrfp1 antibody
    see all

Images

  • ab175125 (5 µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 µg/ml.  Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.

  • All lanes : Anti-ZNRF1 antibody (ab175125) at 1 µg/ml

    Lane 1 : Non-transfected N2a cells
    Lane 2 : Mouse Znrf1 transfected N2a cells
    Lane 3 : Mouse embryo brain lysate
    Lane 4 : Mouse adult brain lysate

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    Primary incubation was 1 hour.

References

ab175125 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175125.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up