Key features and details
- Goat polyclonal to ZNRF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZNRF1 antibody
DescriptionGoat polyclonal to ZNRF1
Specificityab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
- Znrf1 transfected N2a cells, Mouse embryo and adult brain lysates and Mouse cerebral cortex tissue
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab175125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa.
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to substrates. May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin-protein ligase activity.
Tissue specificityExpressed primarily in the nervous system, with expression higher in developing brain relative to adult. Expressed at low levels in testis and thymus.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain is required for E3 ligase activity.
Cellular localizationEndosome. Lysosome. Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Associated with synaptic vesicle membranes in neurons.
- Information by UniProt
- B830022L21Rik antibody
- DKFZp434E229 antibody
- E3 ubiquitin protein ligase ZNRF1 antibody
ab175125 (5 µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 µg/ml. Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.
All lanes : Anti-ZNRF1 antibody (ab175125) at 1 µg/ml
Lane 1 : Non-transfected N2a cells
Lane 2 : Mouse Znrf1 transfected N2a cells
Lane 3 : Mouse embryo brain lysate
Lane 4 : Mouse adult brain lysate
Developed using the ECL technique.
Predicted band size: 24 kDa
Primary incubation was 1 hour.
ab175125 has not yet been referenced specifically in any publications.