Key features and details
- Rabbit polyclonal to ZnT-7
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZnT-7 antibody
See all ZnT-7 primary antibodies
DescriptionRabbit polyclonal to ZnT-7
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: ZnT-7 over-expression - HEK-293T whole cell lysate; IHC-P: Human small intestine tissue; ICC: A431 cells.
Previously labelled as SLC30A7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionSeems to facilitate zinc transport from the cytoplasm into the Golgi apparatus. Partly regulates cellular zinc homeostasis. Required with ZNT5 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and the vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT5 and ZNT6 for the activation of TNAP.
Sequence similaritiesBelongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.
Cellular localizationGolgi apparatus > trans-Golgi network membrane.
- Information by UniProt
- 1810059J10Rik antibody
- 2610034N15Rik antibody
- 4833428C12Rik antibody
All lanes : Anti-ZnT-7 antibody (ab224405) at 0.4 µg/ml
All lanes : ZnT-7 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 42 kDa
Immunohistochemical analysis of human small intestine tissue labeling ZnT-7 in the luminal membrane of glandular cells with ab224405 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling ZnT-7 (green) in Golgi apparatus with ab224405 at 2 µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224405 has not yet been referenced specifically in any publications.