Overview

  • Product name

    Anti-ZO1 tight junction protein antibody
    See all ZO1 tight junction protein primary antibodies
  • Description

    Rabbit polyclonal to ZO1 tight junction protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human ZO1 tight junction protein aa 1670-1720 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    EIYFKVCRDNS ILPPLDKEKG ETLLSPLVMC GPHGLKFLKP VELRLPHCDP


    Database link: Q07157

  • Positive control

    • Human testis, mouse colon, rat brain tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity

    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain

    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human testis tissue labeling ZO1 tight junction protein with ab214228 at 1/250 dilution.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded rat brain tissue labeling ZO1 tight junction protein with ab214228 at 1/100 dilution, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse colon tissue labeling ZO1 tight junction protein with ab214228 at 1/100 dilution.

References

This product has been referenced in:

  • Zhang K  et al. Rumen-derived lipopolysaccharide induced ruminal epithelium barrier damage in goats fed a high-concentrate diet. Microb Pathog 131:81-86 (2019). Read more (PubMed: 30910720) »
  • Hu J  et al. Lactobacillus frumenti Facilitates Intestinal Epithelial Barrier Function Maintenance in Early-Weaned Piglets. Front Microbiol 9:897 (2018). Read more (PubMed: 29867808) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 30°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Apr 07 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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