Anti-ZO1 tight junction protein antibody (ab96587)

Overview

  • Product name
    Anti-ZO1 tight junction protein antibody
    See all ZO1 tight junction protein primary antibodies
  • Description
    Rabbit polyclonal to ZO1 tight junction protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.

  • Positive control
    • WB: 293T, A431, HeLa, HepG2, A549, H1299 and HCT-116 cell lysates; Mouse testis tissue extract; HeLa whole cell extract. IHC-P: Mouse testis and intestine tissues; H661 xenograft tissue. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: 20% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 187 kDa.

Target

  • Function
    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity
    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain
    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization
    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Images

  • All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/3000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 187 kDa

  • Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for ZO1 tight junction protein (green) using ab96587 at 1/200 dilution in ICC/IF. Blue: Hoechst 33342 staining.

  • All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : ZO1 tight junction protein shRNA-transfected HeLa whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 187 kDa

  • Paraffin-embedded H661 xenograft tissue stained for ZO1 tight junction protein using ab96587 at 1/250 dilution in immunohistochemical analysis.

  • Anti-ZO1 tight junction protein antibody (ab96587) at 1/3000 dilution (5% SDS PAGE) + H1299 whole cell lysate
    at 30 µg

    Predicted band size: 187 kDa

  • All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution

    Lane 1 : 293T
    Lane 2 : A431
    Lane 3 : HeLa
    Lane 4 : HepG2

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 187 kDa

  • Immunohistochemical analysis of paraffin embedded mouse intestine tissue labeling ZO1 with ab96587 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin embedded mouse testis labeling ZO1 with ab96587 at 1/500 dilution.

  • ab96587 staining ZO1 tight junction protein in NT2D1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/200) stained green. Nuclei stained blue with Hoechst.

  • Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + Mouse testis tissue extract at 50 µg

    Secondary
    HRP conjugated anti-rabbit IgG

    Predicted band size: 187 kDa

References

This product has been referenced in:
  • Chen H  et al. Inhibitory Effects of Plumbagin on Retinal Pigment Epithelial Cell Epithelial-Mesenchymal Transition In Vitro and In Vivo. Med Sci Monit 24:1502-1510 (2018). Read more (PubMed: 29532788) »
  • Yuan X  et al. Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma. Sci Rep 8:7950 (2018). Read more (PubMed: 29784924) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small intestine)
Permeabilization
No
Specification
Small intestine
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted May 01 2018

Thank you for contacting us.

Unfortunately ab96587 has not been tested in any applications other than WB. It doesn’t mean it won’t work in IHC, but we don’t have data to support it.

If you are interested in partici...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up