Overview

  • Product name

    Anti-ZO1 tight junction protein antibody - C-terminal
    See all ZO1 tight junction protein primary antibodies
  • Description

    Goat polyclonal to ZO1 tight junction protein - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Monkey
  • Immunogen

    Recombinant fragment within Human ZO1 tight junction protein aa 1580-1748 (C terminal). The exact sequence is proprietary. (Purified from E.coli; NP_003248.3).
    Database link: Q07157

  • Positive control

    • Human kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/250.
WB 1/500 - 1/2000. Predicted molecular weight: 195 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity

    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain

    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 µg/ml.

References

This product has been referenced in:

  • Tang H  et al. MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer. Cancer Manag Res 11:201-215 (2019). Read more (PubMed: 30636894) »
  • Huang TW  et al. Chitosan-hyaluronan: promotion of mucociliary differentiation of respiratory epithelial cells and development of olfactory receptor neurons. Artif Cells Nanomed Biotechnol 47:564-570 (2019). Read more (PubMed: 30857434) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Heart
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 17 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Pig Brain Endothelial Cells)
Permeabilization
No
Specification
Pig Brain Endothelial Cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Acetone:Methanol 1:1

Ms. Chloe Whitehouse

Verified customer

Submitted Aug 27 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Pancreas
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Jul 03 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 07 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (kidney)
Permeabilization
Yes - 0.1%tritonX
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Victoria Wu

Verified customer

Submitted Jan 24 2018

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Colonic epithelial cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Colonic epithelial cells
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Methanol

mahesha yatigalpotht

Verified customer

Submitted Nov 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
Yes - 0.5% Triton-X 100
Specification
small intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Oviduct, epithelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
Oviduct, epithelial cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 15 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Primary tracheal epithelial cell)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Primary tracheal epithelial cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Nicky O'Boyle

Verified customer

Submitted Jan 05 2016

Application
IHC - Wholemount
Sample
Mouse Embryo (Mouse embryo tissue)
Specification
Mouse embryo tissue

Patricia Ybot

Verified customer

Submitted Oct 19 2015

1-10 of 12 Abreviews or Q&A

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