Key features and details
- Goat polyclonal to ZO1 tight junction protein - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Dog, Human
- Isotype: IgG
Product nameAnti-ZO1 tight junction protein antibody - C-terminal
See all ZO1 tight junction protein primary antibodies
DescriptionGoat polyclonal to ZO1 tight junction protein - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat, Monkey
Recombinant fragment within Human ZO1 tight junction protein aa 1580-1748 (C terminal). The exact sequence is proprietary. (Purified from E.coli; NP_003248.3).
Database link: Q07157
- WB: MDCK cell lysate. IHC-P: Human kidney tissue. ICC/IF: Primary RPE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/250.|
|WB||1/500 - 1/2000. Predicted molecular weight: 195 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain.
DomainThe second PDZ domain mediates interaction with GJA12.
modificationsPhosphorylated. Dephosphorylated by PTPRJ.
Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
- Information by UniProt
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 µg/ml.
Immunocytochemistry/ Immunofluorescence analysis of primary RPE cells labeling ZO1 tight junction protein with ab190085 at 1/100 dilution.
Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) at 1/2500 dilution + MDCK cell lysate at 50 µg
Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 195 kDa
ab190085 has been referenced in 15 publications.
- Tang H et al. MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer. Cancer Manag Res 11:201-215 (2019). PubMed: 30636894
- Huang TW et al. Chitosan-hyaluronan: promotion of mucociliary differentiation of respiratory epithelial cells and development of olfactory receptor neurons. Artif Cells Nanomed Biotechnol 47:564-570 (2019). PubMed: 30857434
- MohanKumar K et al. A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions. Nat Commun 10:3494 (2019). PubMed: 31375667
- Cuevas E et al. Amyloid Beta 25-35 induces blood-brain barrier disruption in vitro. Metab Brain Dis 34:1365-1374 (2019). PubMed: 31267346
- Zhao Y et al. Peroxisome Proliferator-Activated Receptor ? Agonist Rosiglitazone Protects Blood-Brain Barrier Integrity Following Diffuse Axonal Injury by Decreasing the Levels of Inflammatory Mediators Through a Caveolin-1-Dependent Pathway. Inflammation 42:841-856 (2019). PubMed: 30488141