Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)
Key features and details
- Goat polyclonal to ZO1 tight junction protein - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, IHC-FoFr, IHC-Fr
- Reacts with: Dog, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ZO1 tight junction protein antibody - C-terminal
See all ZO1 tight junction protein primary antibodies -
Description
Goat polyclonal to ZO1 tight junction protein - C-terminal -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IHC-FoFr, IHC-Frmore details -
Species reactivity
Reacts with: Dog, Human
Predicted to work with: Mouse, Rat, Monkey -
Immunogen
Recombinant fragment within Human ZO1 tight junction protein aa 1580-1748 (C terminal). The exact sequence is proprietary. (Purified from E.coli; NP_003248.3).
Database link: Q07157 -
Positive control
- WB: MDCK cell lysate. IHC-P: Human kidney tissue. ICC/IF: Primary RPE cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (7) |
1/50 - 1/250.
|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 195 kDa.
|
IHC-P | (12) |
Use a concentration of 10 µg/ml.
|
IHC-FoFr | (3) |
Use at an assay dependent concentration.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
Notes |
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ICC/IF
1/50 - 1/250. |
WB
1/500 - 1/2000. Predicted molecular weight: 195 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
IHC-FoFr
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. -
Tissue specificity
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. -
Domain
The second PDZ domain mediates interaction with GJA12. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ. -
Cellular localization
Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact. - Information by UniProt
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Database links
- Entrez Gene: 403752 Dog
- Entrez Gene: 7082 Human
- Entrez Gene: 21872 Mouse
- Entrez Gene: 292994 Rat
- Omim: 601009 Human
- SwissProt: O97758 Dog
- SwissProt: Q07157 Human
- SwissProt: P39447 Mouse
see all -
Alternative names
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 µg/ml.
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Immunocytochemistry/ Immunofluorescence analysis of primary RPE cells labeling ZO1 tight junction protein with ab190085 at 1/100 dilution.
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Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) at 1/2500 dilution + MDCK cell lysate at 50 µg
Secondary
Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 195 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab190085 has been referenced in 45 publications.
- Huang LY et al. Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model. Front Cell Neurosci 16:841544 (2022). PubMed: 35308117
- Groestlinger J et al. Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells. Chem Res Toxicol 35:731-749 (2022). PubMed: 35405071
- Hu Q et al. 2-Methoxyestradiol Alleviates Neuroinflammation and Brain Edema in Early Brain Injury After Subarachnoid Hemorrhage in Rats. Front Cell Neurosci 16:869546 (2022). PubMed: 35558877
- Zhou X et al. Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets. Gut Microbes 14:2091369 (2022). PubMed: 35758253
- Groestlinger J et al. Combinatory Exposure to Urolithin A, Alternariol, and Deoxynivalenol Affects Colon Cancer Metabolism and Epithelial Barrier Integrity in vitro. Front Nutr 9:882222 (2022). PubMed: 35811943