RecombinantRabMAb

Recombinant Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19945-224] to ZO1 tight junction protein
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZO1 tight junction protein antibody [EPR19945-224]
    See all ZO1 tight junction protein primary antibodies

  • Description

    Rabbit monoclonal [EPR19945-224] to ZO1 tight junction protein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human ZO1 tight junction protein aa 1-300. The exact sequence is proprietary.
    Database link: Q07157

  • Positive control

    • IHC-P: Human bronchial epithelium, breast cancer and kidney tissue. Mouse kidney tissue. Rat spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19945-224

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or WB.

    • Target

    • Function

      The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

    • Tissue specificity

      The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

    • Sequence similarities

      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 3 PDZ (DHR) domains.
      Contains 1 SH3 domain.
      Contains 1 ZU5 domain.

    • Domain

      The second PDZ domain mediates interaction with GJA12.

    • Post-translational
      modifications

      Phosphorylated. Dephosphorylated by PTPRJ.

    • Cellular localization

      Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
    • Information by UniProt

    • Database links

      • Alternative names

        • Tight junction protein 1 antibody
        • Tight junction protein ZO-1 antibody
        • Tight junction protein ZO1 antibody
        • TJP1 antibody
        • zo-1 antibody
        • Zo1 antibody
        • ZO1_HUMAN antibody
        • Zona occludens 1 antibody
        • Zona occludens 1 protein antibody
        • Zona occludens protein 1 antibody
        • Zonula occludens 1 protein antibody
        • Zonula occludens protein 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Immunohistochemical analysis of paraffin-embedded human bronchial epithelium tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in epithelial and endothelial cells of human bronchial epithelium (PMID: 17585317) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in glomerulus of human kidney (PMID: 18618131) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in breast cancer (PMID: 9846967) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in glomerular of mouse kidney (PMID: 18618131) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of rat spleen (PMID: 17585317) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

        Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ZO1 tight junction protein with ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in lymphocytes of human tonsil (PMID: 17585317) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)
        Anti-ZO1 tight junction protein antibody [EPR19945-224] (ab221546)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab221546? Please let us know so that we can cite the reference in this datasheet.

      ab221546 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ZO1 tight junction protein antibody [EPR19945-224]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Chicken Tissue sections (Ileum)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
      Permeabilization
      No
      Specification
      Ileum
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 30 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ZO1 tight junction protein antibody [EPR19945-224]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Pig Tissue sections (Kidney, liver, spleen, skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
      Permeabilization
      No
      Specification
      Kidney, liver, spleen, skin
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
      Fixative
      10% NBF
      Read More

      Mr. Victor Bernal

      Verified customer

      Submitted Mar 18 2020

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