Overview

  • Product name

    Anti-ZO1 tight junction protein antibody [mAbcam 61357] (FITC)
    See all ZO1 tight junction protein primary antibodies
  • Description

    Mouse monoclonal [mAbcam 61357] to ZO1 tight junction protein (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to ZO1 tight junction protein aa 750-850 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Flow Cyt: MCF7 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 61357
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

Target

  • Function

    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity

    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain

    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Images

  • Overlay histogram showing MCF7 cells stained with ab150266 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150266, 0.1μg/1x106 cells) for 30 min at 22ºC.

    Isotype control antibody (black line) was mouse IgG1 [ICIG1] FITC (ab91356) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Liu L  et al. Angiotensin II inhibits the protein expression of ZO-1 in vascular endothelial cells by downregulating VE-cadherin. Mol Med Rep 18:429-434 (2018). Read more (PubMed: 29749551) »
  • Li Y  et al. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways. Biomed Res Int 2018:3242574 (2018). Read more (PubMed: 29850504) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Chicken Tissue sections (Hindbrain section)
Specification
Hindbrain section
Permeabilization
Yes - PBS + 0.1% Tween20
Fixative
Paraformaldehyde

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Verified customer

Submitted Dec 04 2013

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