Product nameAnti-zona radiata antibody [MN-7F2]
See all zona radiata primary antibodies
DescriptionMouse monoclonal [MN-7F2] to zona radiata
SpecificityThis antibody binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the g monomer.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Rainbow trout
Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).
- Plasma from 17ß-estradiol treated Salmon.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesAffinity purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab36802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/1000. Detects a band of approximately 45 kDa.
The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceZona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
- SwissProt: Q90XC3_ONCMY Rainbow trout
- ZRP antibody
Coating: Plasma (diluted 1:1000) from 17ß-estradiol treated (E2) or control (C) fish.
Lane 1 : Molecular weight standards
Lanes 2-10 : Anti-zona radiata antibody [MN-7F2] (ab36802) at 1/500 dilution
Lane 2 : Purified Zrp, [alpha, beta and gamma].
Lane 3 : Plasma from 17ß-estradiol treated Salmon at 1/200 dilution
Lane 4 : Plasma from control Salmon at 1/200 dilution
Lane 5 : Plasma from 17ß-estradiol treated Arctic char at 1/200 dilution
Lane 6 : Plasma from control Arctic char at 1/200 dilution
Lane 7 : Plasma from 17ß-estradiol treated Turbot at 1/200 dilution
Lane 8 : Plasma from control Turbot at 1/200 dilution
Lane 9 : Plasma from 17ß-estradiol treated Cod at 1/200 dilution
Lane 10 : Plasma from control Cod at 1/200 dilution
ab36802 has not yet been referenced specifically in any publications.